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computer assisted analysis  (Olympus)


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    Structured Review

    Olympus computer assisted analysis
    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
    Computer Assisted Analysis, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 10297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/computer-assisted+image+analysis/cellSens+Imaging+Software/pmc12939340-97-8-16
    Average 99 stars, based on 10297 article reviews
    computer assisted analysis - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Stefin B and Cystatin C Deficiency Suppresses Tumor Growth and Alters Tumor Microenvironment in a Breast Cancer Model"

    Article Title: Stefin B and Cystatin C Deficiency Suppresses Tumor Growth and Alters Tumor Microenvironment in a Breast Cancer Model

    Journal: Cells

    doi: 10.3390/cells15040360

    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
    Figure Legend Snippet: Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Techniques Used: Immunodetection, TUNEL Assay

    Related Articles

    Imaging:

    Article Title: Variation in Brain Morphology of Intertidal Gobies: A Comparison of Methodologies Used to Quantitatively Assess Brain Volumes in Fish.
    Article Snippet: .. Between imaging sessions, histological analysis and micro-CT analysis brains were stored in 0.1 M PBS buffer at 4 ° C. Volumetric Analysis Using Ellipsoid Model Digital images were acquired of the brains’ dorsal, ventral and lateral views using a computer-assisted video image analysis system, employing an Olympus SZX16 research stereo microscope equipped with a ColourView digital camera (ColourView IIIu). .. Images were acquired using analySIS docu version 5.0 (Olympus Soft Imaging Solutions, 2007).

    Micro-CT:

    Article Title: Variation in Brain Morphology of Intertidal Gobies: A Comparison of Methodologies Used to Quantitatively Assess Brain Volumes in Fish.
    Article Snippet: .. Between imaging sessions, histological analysis and micro-CT analysis brains were stored in 0.1 M PBS buffer at 4 ° C. Volumetric Analysis Using Ellipsoid Model Digital images were acquired of the brains’ dorsal, ventral and lateral views using a computer-assisted video image analysis system, employing an Olympus SZX16 research stereo microscope equipped with a ColourView digital camera (ColourView IIIu). .. Images were acquired using analySIS docu version 5.0 (Olympus Soft Imaging Solutions, 2007).

    Microscopy:

    Article Title: Variation in Brain Morphology of Intertidal Gobies: A Comparison of Methodologies Used to Quantitatively Assess Brain Volumes in Fish.
    Article Snippet: .. Between imaging sessions, histological analysis and micro-CT analysis brains were stored in 0.1 M PBS buffer at 4 ° C. Volumetric Analysis Using Ellipsoid Model Digital images were acquired of the brains’ dorsal, ventral and lateral views using a computer-assisted video image analysis system, employing an Olympus SZX16 research stereo microscope equipped with a ColourView digital camera (ColourView IIIu). .. Images were acquired using analySIS docu version 5.0 (Olympus Soft Imaging Solutions, 2007).



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    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor <t>using</t> <t>computer-assisted</t> data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.
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    Image Search Results


    Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Journal: Cells

    Article Title: Stefin B and Cystatin C Deficiency Suppresses Tumor Growth and Alters Tumor Microenvironment in a Breast Cancer Model

    doi: 10.3390/cells15040360

    Figure Lengend Snippet: Effect of stefin B and cystatin C deficiency on proliferation and cell death. ( A ) Cell proliferation in primary tumors was determined through immunodetection of Ki67 in PyMT;WT and PyMT;DKO mice at 14 weeks of age. Representative images and quantification of Ki67-positive cells as a percentage of total cells in PyMT;WT (n = 11) and PyMT;DKO (n = 10) tumors. Ki67 index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test. ( B ) Cell death in primary tumors from PyMT;WT and PyMT;DKO mice at 14 weeks of age was determined through TUNEL assays. Representative images and quantification of TUNEL-positive cells as a percentage of total cells in PyMT;WT (n = 9) and PyMT;DKO (n = 8) tumors. The TUNEL+ index was calculated from 10 high-power fields per tumor using computer-assisted data analysis in ImageJ. Differences were analyzed using Student’s t -test.

    Article Snippet: The average size of metastases was quantified using computer-assisted analysis with CellSens Imaging software (Version 1.17; Olympus, Tokyo, Japan).

    Techniques: Immunodetection, TUNEL Assay